Putative transcription factors show cyclic RNA abundance patterns.

<p>Cell cycle expression profiles were assessed for 68 predicted AP2 domain genes <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012354#pone.0012354-Altschul1" target="_blank">[49]</a> and 9 genes containing C2H2 Zn-finger domains (TGME49_103050, _121330, _101340, _086710, _006650, _002470, _059650, _023570, _111450) found in the <i>Toxoplasma</i> genome. (<b>A</b>) Maximum expression of RNA polymerases was tightly clustered in the G1-subtranscriptome along with a few general transcription factors. By contrast, the times of peak expression for 24 AP2 (see full list <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012354#pone.0012354.s001" target="_blank">Table S1</a>) and four activator Zn-finger domain genes were distributed across the tachyzoite division cycle (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012354#pone.0012354.s002" target="_blank">Table S2</a>). In comparison, mRNAs encoding RNA polymerases showed peak expression clustered in G1. Relatively few general transcription factors and less than a third of known chromatin remodelers <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012354#pone.0012354-Dixon1" target="_blank">[87]</a> also have cyclical patterns (see also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012354#pone.0012354.s002" target="_blank">Table S2</a>). (<b>B</b>) Spline model curves for selected cell cycle AP2 mRNAs demonstrate relative abundance and time shifts indicating that the expression of these factors follows a serial order with peaks at different cell cycle stages.</p>