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Purification of recombinant Y. pestis MEP synthase.

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posted on 2014-08-29, 02:58 authored by Amanda Haymond, Chinchu Johny, Tyrone Dowdy, Brandon Schweibenz, Karen Villarroel, Richard Young, Clark J. Mantooth, Trishal Patel, Jessica Bases, Geraldine San Jose, Emily R. Jackson, Cynthia S. Dowd, Robin D. Couch

A Coomassie stained SDS-PAGE shows two lanes of purified His-tagged MEP synthase alongside a molecular weight marker (MW). His-tagged MEP synthase has a predicted molecular weight of 46.7 kDa. The typical yield of purified protein averaged 30 mg per 1 L shake flask.

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