Purification of YlLac from the culture broth of P. pastoris KM71H cells.
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posted on 2015-03-17, 04:24 authored by Dayanand Kalyani, Manish Kumar Tiwari, Jinglin Li, Sun Chang Kim, Vipin C. Kalia, Yun Chan Kang, Jung-Kul LeeP. pastoris KM71H was grown for 7 days in BMM medium containing 0.2 mM CuSO4 (30°C; 200 rpm). YlLac protein was purified from the culture broth as indicated. The activity was monitored by oxidation of ABTS under standard assay conditions (see materials method). The expression level of YlLac in P. pastoris was 4.9 mg/L.
Purification of YlLac from the culture broth of P. pastoris KM71H cells.
