Figure_4.tif (200.86 kB)
PtdIns5P levels and ATX1 activity in AtMTM1 mutant cells.
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posted on 2013-02-20, 22:56 authored by Ivan Ndamukong, David R. Jones, Hanna Lapko, Nullin Divecha, Zoya AvramovaPtdIns5P levels in fresh and dehydrated wild type leaves, in OX-AtMTM1 leaves, and in leaves from homozygous mtm1 plants (panel a). Relative WRKY70 transcripts in non-stressed and in stressed wild-type, OX-AtMTM1, and mtm1 mutant cells, quantified by real-time PCR (panel b). Quantitative PCR of ChIP assays of H3K4me3 methylation in wild-type, OX-ATMTM1 and mtm1 mutant chromatins in fresh leaves and in leaves after dehydration-stress (panel c). Relative enrichment in WRKY70-specific fragments vs input DNA.
