Protein staining and reactivity with serum derived from vaccinated mice revealed differences among vaccine extracts.

Twenty μg of protein extracts, prepared from whole LVS, HK-LVS, LVS-G, and LVS-R, were loaded on SDS-PAGE in reducing conditions and stained with Red Ponceau (Panel A). Following Ponceau staining, reactivity of protein extracts was analyzed by blotting the membranes with pooled sera derived from LVS vaccinated (Panel B) and from HK-LVS vaccinated BALB/cByJ mice (Panel C). Results shown are from one representative experiment of four independent experiments of similar design and outcome.