Protein expression of myosin IC isoforms A and B in mammalian cell lines.

<p>Detection of myosin IC isoforms A and B protein in various mammalian cell lines by immunoblot analysis using antibodies specific to myosin IC isoforms A, isoform B, SV40 large T-antigen (TAg) and actin (control). <b>A)</b> Schematic of myosin IC isoform specific sequences and recognition site of antibodies. Depicted is the 5′ region of the mammalian <i>MYOIC</i> gene with the exons that code for isoform specific N-terminal peptides. The translation start sites for the isoforms are indicated as are the N-terminal amino acid sequences. Underlined are the peptide sequences used as immunogen to create isoform-specific antibodies. <b>B)</b> Representative immunoblot of cell extracts that were analyzed using the indicated antibodies. Relevant molecular weight markers are indicated on the left in kD. <b>C)</b> Histogram presenting the average densitometric intensity of myosin IC isoform A expression normalized to actin. <b>D)</b> Histogram presenting the average densitometric intensity of isoform B expression normalized to actin. Myosin IC isoform A protein is highly expressed in COS-7 cells and the human and mouse prostate cancer cell lines PC-3 and TRAMP-C2. In contrast, myosin IC isoform B protein is expressed at comparable levels in all analyzed cell lines. Results are presented as means ± standard deviation; n = 3 independent experiments.</p>