Protein content of T1α in the PCLS of wild type mice and the alveolar epithelial cell line E10.
Immunoblots of PCLS and E10 experiments without (A) and with AMJ2-C11 macrophages (B). For PCLS the concentration of nano-silica was 1600 µg/ml and for E10 experiments nano-silica concentrations ranging from 50 to 200 µg/cm2 flask area were used. Data of each silica group was normalized to the control. Incubation time of PCLS was 72 hours and for E10 experiments 48 hours. γ-Tub is the loading control, n = 6–7.