Protein and mRNA expression levels of IL-17, IL-22, IL-23, GM-CSF, and IFN-γ.

(A) Protein expression levels of IL-17, IL-22, IL-23, GM-CSF, and IFN-γ. GAPDH was used as a loading control for Western blot analysis. Data are mean ± SD of three independent experiments. Densitometric analysis of each protein level was calculated from the average of three experiments. Each value was expressed as the ratio of the measured protein to GAPDH level (P<0.001). (B) mRNA expression levels of IL-17, IL-22, IL-23, GM-CSF, and IFN-γ from colon cancer tissues performed by RT-PCR analysis. 5 µg of total RNA was isolated from cancer tissues. Values represent the mean ± SD of three independent experiments.