Proliferation of c-kit<sup>+</sup> cells in the lungs of AMD3100-treated SU5416/chronic hypoxia (SuHx) animals.

<p>(<b>A</b>) Representative optical sections (confocal microscopy) demonstrate c-kit<sup>+</sup> von Willebrand Factor<sup>+</sup> (vWF<sup>+</sup>) proliferating cell nuclear antigen<sup>+</sup> (PCNA<sup>+</sup>) cells (upper row) or c-kit<sup>+</sup> α-smooth muscle actin<sup>+</sup> (α-SMA<sup>+</sup>) PCNA<sup>+</sup> cells (lower row) in pulmonary arteries of SuHx + vehicle and SuHx + AMD3100 treated animals. The inserts show a triple positive cell (arrow) in more detail. Please note that the bright white stained dots indicate cells with very strong PCNA staining. Nuclear counterstaining: 4',6-diamidino-2-phenylindole (DAPI). Magnification: 630×. Scale bar: 20 µm. (<b>B-C</b>) Quantification of the number of c-kit<sup>+</sup>, c-kit<sup>+</sup> vWF<sup>+</sup> and c-kit<sup>+</sup> α-SMA<sup>+</sup>, as well as PCNA<sup>+</sup>, c-kit<sup>+</sup> PCNA<sup>+</sup>, c-kit<sup>+</sup> vWF<sup>+</sup> PCNA<sup>+</sup> and c-kit<sup>+</sup> α-SMA<sup>+</sup> PCNA<sup>+</sup> cells in and around the pulmonary arteries of SuHx + vehicle and SuHx + AMD3100 animals. n = 3 animals per group. * <i>P</i><0.05 and ** <i>P</i><0.01.</p>