figshare
Browse
Figure_8.tif (1.76 MB)

Proliferation of c-kit+ cells in the lungs of AMD3100-treated SU5416/chronic hypoxia (SuHx) animals.

Download (0 kB)
figure
posted on 2014-02-24, 03:32 authored by Daniela Farkas, Donatas Kraskauskas, Jennifer I. Drake, Aysar A. Alhussaini, Vita Kraskauskiene, Harm J. Bogaard, Carlyne D. Cool, Norbert F. Voelkel, Laszlo Farkas

(A) Representative optical sections (confocal microscopy) demonstrate c-kit+ von Willebrand Factor+ (vWF+) proliferating cell nuclear antigen+ (PCNA+) cells (upper row) or c-kit+ α-smooth muscle actin+ (α-SMA+) PCNA+ cells (lower row) in pulmonary arteries of SuHx + vehicle and SuHx + AMD3100 treated animals. The inserts show a triple positive cell (arrow) in more detail. Please note that the bright white stained dots indicate cells with very strong PCNA staining. Nuclear counterstaining: 4',6-diamidino-2-phenylindole (DAPI). Magnification: 630×. Scale bar: 20 µm. (B-C) Quantification of the number of c-kit+, c-kit+ vWF+ and c-kit+ α-SMA+, as well as PCNA+, c-kit+ PCNA+, c-kit+ vWF+ PCNA+ and c-kit+ α-SMA+ PCNA+ cells in and around the pulmonary arteries of SuHx + vehicle and SuHx + AMD3100 animals. n = 3 animals per group. * P<0.05 and ** P<0.01.

History