Progressive decrease in the erythrocytes sCLU levels during <i>ex vivo</i> cellular senescence.

<p>Representative sCLU immunoblot (A<sub>1</sub>) and densitometric analysis (A<sub>2</sub>) of membrane (A<sub>1</sub>, upper panels) and cytosol (A<sub>1</sub>, lower panels) preparations derived from leukoreduced RBCs units (N = 4) stored for the indicated duration in SAGM solution. (B) Comparative densitometric analysis of immunoblots (not shown) of sCLU relative membrane levels in RBCs stored either in SAGM (N = 4; max 42 days of storage) or autologous plasma (Au-Pl) (N = 3; max 35 days of storage). Probing with anti-4.1R and anti-peroxiredoxin-2 (Prx2) was used as a protein loading reference. Shown densitometric data (mean values of at least two independent experiments) indicate relative proportion against a loading reference followed by normalization against the samples stored for a short period (e.g. 4 days); error bars indicate ± standard deviation. Asterisks and dots indicate difference of each day of storage vs. day 4 and SAGM vs. Au-Pl respectively, at significance level of p<0.05.</p>