Production of 11-cis retinol by Lamprey RPE65 in HEK293F cells.

A: Normal-phase HPLC of retinol isomers from saponified retinyl esters isolated from HEK293F cells expressing Lamprey RPE65 and bovine LRAT (blue trace). B: Normal-phase HPLC of retinol isomers from saponified retinyl esters isolated from HEK293F cells expressing Lamprey RPE65 with Lamprey LRAT (red trace) or only Lamprey LRAT (green trace).