Primers used in high-efficiency thermal asymmetric interlaced PCR.

<p><sup>a</sup> DTn10AP1, DTn10AP2 and DTn10AP3 are primers specific to transposon gene and were used to retrieve the disrupted genes in mutants; LAD1-1, LAD1-2, LAD1-3, LAD1-4 and LAD1-5 are arbitrary primers which were designed based on the report [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0122741#pone.0122741.ref027" target="_blank">27</a>]; the remaining primers were designed based on the intermediate sequences of the disrupted genes in mutants B1, B6 and B19, respectively, and used to amplify for 3’-region of <i>sdmT</i> gene (Fsdmt1 (1st), Fsdmt2 (2nd), Fsdmt3 (3rd)), 5’-region of <i>nhaD</i> gene (RnhaD1 (1st), RnhaD2 (2nd), RnhaD3 (3rd)), 3’-region of <i>nhaD</i> gene (FnhaD1 (1st), FnhaD2 (2nd), FnhaD3 (3rd)), 5’-region of <i>karI</i> gene (Rkari1 (1st), Rkari2 (2nd), Rkari3 (3rd)), and 3’-region of <i>karI</i> gene (Fkari1 (1st), Fkari2 (2nd), Fkari3 (3rd)).</p><p><sup>b</sup> V = A/C/G; N = A/C/G/T; B = C/G/T; H = A/C/T; D = A/G/T.</p><p>Primers used in high-efficiency thermal asymmetric interlaced PCR.</p>