Primer schedule.

<p>A summary of primer names, for/rev primer sequences (5′ to 3′), PCR product size (amplicons in bps), annealing conditions and Genebank accession numbers of each gene investigated. F: forward primer; R: reverse primer.</p>a<p>PCR primers were designed by Primer3 software (genome.wi.mit.edu/cgi-bin/primer/primer3_<a href="http://www.cgi" target="_blank">www.cgi</a>) and synthesized by MWG (mwg.com/; Ebersberg, Germany).</p>b<p>The RNAi were designed by Invitrogen website (rnaidesigner.invitrogen.com/).</p>c<p>Amplification parameters were as follows: 37 cycles of 30 s/94°C, 25s/specific Ta, 30 s/72°C, preceded by 15 m/95°C hot start polymerase activation and followed by fluorescence monitoring during linear transition from 55–90°C, 0.01°C for 0.3 sec and further 5 m/72°C incubation.</p>