Prediction of molecular forces from in vitro measurements.
The experimental results from Klotzsch 2009 were scaled using the UV absorption measurements of Fn concentration at 280 nm. The load on individual molecules in the equal loading configuration (A,B) was calculated by dividing the measured fiber tension by the calculated number of fibrils (1.65e5 fibrils). The loads in the disparate loading condition (C) were calculated by dividing the measured fiber tension by the number of disparately loaded fibrils (5.50e4 fibrils) then solving for the forces required to maintain the ratio between molecular forces found in the disparate loaded simulation (D). In the disparate loading condition there are two distinct populations of molecular forces. The more extended molecules (i) are under a higher load while the pair of molecules connected in series (ii) is shielded from force.