Pooled siRNAs demonstrate greater knockdown efficacy than single siRNAs.

<p>Comparison of the ability of transfected pooled vs. single siRNAs to A. knockdown target gene mRNA levels and B. reduce G6PC mRNA levels in AH-G6PC cells incubated with Dex/cAMP and 10 nM insulin for 6 h. Black bars are pooled siRNA samples; grey bars are single siRNA samples. Data are presented as fold changes relative to mRNA expression in siControl-transfected cells and are the means of 3 independent experiments for A and B.</p>