Plasmid maintenance in <i>S. typhimurium</i> using the <i>glmS</i> system <i>in vitro</i>.

<p>(A) GlmS<sup>−</sup> mutant (SKS1001) salmonellae carrying <i><sup>s.t</sup>GlmS<sup>+</sup>p</i> were subcultured (1/1000) in minimal media (closed circles) or media supplemented with 0.2% GlcNAc (open circles) every 12 hrs. The fraction of bacteria carrying <i><sup>s.t</sup>GlmS<sup>+</sup>p</i> was determined on the indicated days by the plating method. (B) The same was done with wild-type <i>Salmonellae</i> (SCH2005) in the absence (open circles) or presence (closed circles) of ampicillin.</p>