Plasma membrane and protrusion stiffness and viscosity.

Plasma membrane cholesterol concentration in control cells is 7.54 pmol/µg protein. Lat-A represents treatment of the cells with Latrunculin-A in order to disrupt F-actin polymerization.

Membrane tether effective viscosity was not determined at 5.68 pmol/µg protein cholesterol concentration, but its value under cholesterol depleted conditions at 6.6±0.3 pmol/µg protein was ≈2.1±0.4 pN.s/µm [13].