Phylogenetic tree of class I fumarases and related proteins.

The tree is based on amino acid sequence analysis of N-terminal part of class I fumarases consisting of one subunit (marked with”*” on the tree) and α-subunits of heterodimeric fumarases/ mesaconases/ (2R,3R)-tartrate dehydratases. (2R,3R)-Tartrate dehydratases are shown in blue, enzymes functioning in vivo as mesaconases in red (functions confirmed experimentally or based on the co-localization with glutamate fermentation genes). Tree topography and evolutionary distances are given by the neighbor-joining method with Poisson correction. The scale bar represents a difference of 0.1 substitutions per site. Numbers at nodes indicate the percentage bootstrap values for the clade of this group in 1000 replications. Only values above 70% were considered.