Figure_1.tif (488.72 kB)
Photoactivation of mt-PAGFP.
figure
posted on 2013-12-06, 03:31 authored by Guo Luo, Jianxun Yi, Changling Ma, Yajuan Xiao, Frank Yi, Tian Yu, Jingsong ZhouSimultaneous recording of TMRE (a1) and mt-PAGFP (a2) images. Before photoactivation, there is no GFP fluorescence of mt-PAGFP detected in a2. A small area (~ 10 X 10 µm2) of the fiber was photoactivated by a 364 nm laser (b). After photoactivation, the fiber was imaged again simultaneously for both TMRE (c1) and mt-PAGFP (c2) at the fiber region including the area with photoactivated GFP. Note the visible GFP fluorescence of mt-PAGFP in c2 recorded at the same laser intensity as in a2.