Phenotypic assessment of memory conventional and regulatory CD4⁺ T-cell subsets in circulating lymphocytes of healthy donors and EOC patients.

A. CD4⁺ T cells were stained with anti-FOXP3, -CD25, -CD45RA and CD127 antibodies and analyzed by flow cytometry. Expression of CD25 and CD127 defines 3 populations of memory (CD45RA⁻) CD4⁺ T cells: conventional CD25⁻CD127⁺ and CD25⁻CD127⁻ and Treg CD25⁺CD127⁻ (left dot plot, numbers correspond to the proportion of each subset among memory CD4⁺ T cells). Histograms show the expression of FOXP3 in the defined memory CD4⁺ T-cell subsets. B. The proportion of conventional CD25⁻CD127⁺ and CD25⁻CD127⁻ and Treg CD25⁺CD127⁻ subsets, defined in A, among memory CD4⁺ T cells of healthy donors (HD, n = 27) and patients (P, n = 18).