Pgp-GFP fusion protein expression in hCMEC/D3 cells. A:

Simulated conventional microscopy image of Pgp-GFP in hCMEC/D3 cells (by convolving a super-resolution image with a Gaussian function with a standard deviation of 200 nm). A conventional microscopy image was not acquired to prevent bleaching. B: Super-resolution localization microscopy image of the same cell. Each molecule is convolved with a Gaussian function with a standard deviation equal to its localization accuracy. Some Pgp-GFP molecules could be resoluted with a localization accuracy of approximately 42 nm (asterisk). C–E: Three selected images from a total of 20 equivalent images of hCMEC/D3 cells which express Pgp-GFP.