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Percentage of cells stained γ-H2AX positive after irradiation at 8.75 Gy.

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posted on 2013-02-19, 20:02 authored by Seog-Young Kim, Juong G. Rhee, Xinxin Song, Edward V. Prochownik, Douglas R. Spitz, Yong J. Lee

Kinetics of γ-H2AX foci removal after irradiation. Non-stem and CSC-like MDA-MB-435 and MDA-MB-231 cells were irradiated at 8.75 Gy. Various times (0.5–12 hr) after irradiation, cells were fixed and immunostained with anti-phospho-H2AX antibody. Nuclei containing at least six fluorescent foci were considered positive and percentage of cells stained γ-H2AX positive was determined. Error bars represent standard error from the mean for three separate experiments.

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