Per cell analysis of the probabilistic expression of <i>bcpA</i>.

<p>A) P<i><sub>bcpA</sub></i>-<i>lacZ</i> reporter strain plated on M63 minimal medium agar supplemented with X-gal. B) Confocal microscopy of <i>gfp</i> reporter strains, P<i><sub>bcpA</sub></i>-<i>gfp</i>, P<sub>S12</sub>-<i>gfp</i>, and E264Km<sup>R</sup> (vector), cultured overnight in liquid medium. C) Representative flow cytometry analysis of <i>gfp</i> reporter strains, P<i><sub>bcpA</sub></i>-<i>gfp</i>, P<sub>S12</sub>-<i>gfp</i>, and E264Km<sup>R</sup> (vector), cultured overnight in liquid medium. The dot plot (top left panel) of side scatter intensity (SS INT) vs. forward scatter intensity (FS INT) shows the events corresponding to <i>B. thailandensis</i> that were gated on (“Gate”) for subsequent analysis. Histograms show the number of GFP-positive events (Count) vs. relative fluorescence intensity (FL1 INT) for each reporter strain, and the percent of GFP-positive bacteria detected in each culture is indicated.</p>

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