Pathogens that give rise to lethal infections in vivo elicit weak responses from AM.

(A) AM from C57BL/6 mice or cynomolgus macaques were exposed to the indicated pathogens. Supernatants were collected at 0 hr and 24 hr following exposure and examined for the production of TNF and IL-1β by Luminex as described in the Methods. Results are expressed as the mean ± SEM of 3–8 independent experiments. Statistics were performed using one way ANOVA with a Tukey post test. P values: * <0.05, ** <0.01, *** <0.001. (B) Hierarchical Clustering of differentially expressed (DE) genes from murine (1383 genes) and macaque (1387 genes) AM following exposure to the indicated pathogens. The dendrograms were generated as described in Methods. The numbers in the dendrograms represent the frequency with which a particular node was seen in 1000 permutations of the algorithm and gives a level of confidence in the observed structure. (C) Resulting clusters of Softclust analysis over DE genes from all infections in both species. Marked in red are genes that changed cluster assignment following an intermediate orthology reward compared to no-reward run. (D) Infections co-clustering across species in no-reward and intermediate reward settings. Co-clustering increased 82% on average in the rewarded run.