Pacsin-PRD complex formation.

GST pull-down experiments were carried out by using wild-type and mutant forms of GST-PRD to examine their interactions with pacsin-1. Complexes were eluted and analyzed by SDS-PAGE and Coomassie-staining. Bait proteins: wild-type GST-PRD (PRD^wt), GST-PRD^trunc1 (tr1), GST-PRD^trunc2 (tr2), GST-PRD^ArgKO1 (KO1), GST-PRD^ArgKO2 (KO2), GST-PRD^ArgKO3 (KO3) and GST (negative control).