P-HDL plotted as a function of cf Hb: Plate vs. HPLC Assay.

For plate assay, control AA p-HDL data (* RFU/µg HDL_c⋅min) were plotted as open circles and SCD p-HDL data were plotted as open squares. For HPLC assay, controls (AA, meaning homozygous for hemoglobin AA) p-HDL data (* RFU/apoA-I (µg/mL)) were plotted as closed circles and SCD p-HDL data were plotted as closed squares. (y = ax+b; Plate assay: y = 0.03004x+3.554; HPLC assay: y = 0.01688x+0.84140). These data showed that p-HDL isolated by apo B precipitation was more sensitive to the effects of cf Hb and was more variable than p-HDL determined by the AE-HPLC-PCR assay.