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PTEN reconstitution enhances Chordoma cell sensitivity PDGFR inhibition

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posted on 2015-08-06, 03:18 authored by Dae-Hee Lee, Ying Zhang, Amin B. Kassam, Myung-Jin Park, Paul GardnerPaul Gardner, Daniel Prevedello, Stephanie Henry, Craig Horbinski, Jan H. Beumer, Hussein Tawbi, Brian J. Williams, Mark E. Shaffrey, Merrill J. Egorin, Roger Abounader, Deric M. Park

. (A and B) Representative apoptosis assays for (A) C18 cell line (PTEN +/-) and (B) UCH1 cell line (PTEN +/-), respectively, assessed by Annexin-V and 7-AAD florescence demonstrating significantly higher rates of apoptosis with restoration of PTEN and administration of PDGFR inhibitor compared with control. *P<0.05, Two sample independent student’s t test. This is representative of three independent experiments. (C and D) Representative matrigel invasion assays with the C18 cell line (PTEN +/-) and UCH1 cell line (PTEN +/-), respectively, demonstrating significant decrease in invasion in response to PDGF inhibition with restoration of PTEN expression. These images are representative of 3 independent experiments. *P<0.05, Two sample independent student’s t test.

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