PTEN reconstitution enhances Chordoma cell sensitivity PDGFR inhibition
. (A and B) Representative apoptosis assays for (A) C18 cell line (PTEN +/-) and (B) UCH1 cell line (PTEN +/-), respectively, assessed by Annexin-V and 7-AAD florescence demonstrating significantly higher rates of apoptosis with restoration of PTEN and administration of PDGFR inhibitor compared with control. *P<0.05, Two sample independent student’s t test. This is representative of three independent experiments. (C and D) Representative matrigel invasion assays with the C18 cell line (PTEN +/-) and UCH1 cell line (PTEN +/-), respectively, demonstrating significant decrease in invasion in response to PDGF inhibition with restoration of PTEN expression. These images are representative of 3 independent experiments. *P<0.05, Two sample independent student’s t test.