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PRV load and antiviral-related gene expression in Atlantic salmon following injection challenge.

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posted on 2016-01-18, 14:26 authored by Kyle A. Garver, Stewart C. Johnson, Mark P. Polinski, Julia C. Bradshaw, Gary D. Marty, Heindrich N. Snyman, Diane B. Morrison, Jon Richard

The relative individual threshold cycles (Ct) and treatment mean (horizontal bar) obtained by qPCR analysis for all blood and anterior kidney samples obtained during 24 weeks post challenge (wpc). Mean copy number of PRV L1 genomic sequence per 1 μg extracted RNA is also provided in time course, as well as the corrected normalized relative quantity (CNRQ) of Mx antiviral mRNA transcripts for each of three treatment groups at either 1 or 2 wpc for both peripheral blood and anterior kidney tissues. For gene expression analyses, significant (* p<0.05; ** p<0.01; *** p<0.001) differences in expression between treatment groups are indicated at each time point.

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