PP2A and PP1 are translocated from the cytosol to lipid droplets in adipocytes in response to adrenaline stimulation.

<p>(A–C) Translocation of PP2A (A, B) and PP1 (A, C) to the lipid droplet fraction in response to stimulation with 1 µM adrenaline (Adrn). The bar graph shows the amount of PP2A (B) and PP1 (C) in fat (black) and sup (white) fractions, respectively. A typical image from four independent experiments is shown (A). (D, E) Phosphatase activity in floating fat-cake fraction. Phosphatase activities of PP2A (D, n = 4) and PP1 (E, n = 5) were measured using floating fat-cake fractions from WT and <i>PRIP</i>-DKO explants treated with or without 1 µM adrenaline. The data represent mean ±SEM; *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 and n.s. (not significant) versus the corresponding values for paired bars connected by solid and dotted lines (B–E).</p>