PELP1 localizes to the nucleolus.

(A) MCF7 and ZR-75 cells were serum starved for 2 days and stimulated with 10% serum for 12 h. PELP1 (green) localization was visualized by using confocal microscopy. (B) HeLa cells were grown in 10% serum and the localization of PELP1 (green), the nucleoli marker nucleolin (red) and colocalization (yellow) was analyzed by using confocal microscopy. (C) PELP1 localization was analyzed by using a control peptide or PELP1 epitope-specific peptide adsorbed antibody. (D) HeLa cells were transfected with either control or PELP1-specific siRNA and the localization of PELP1 (green) was analyzed by using confocal microscopy. (E) HeLa cells were fractionated into cytoplasm, nucleoplasm and nucleoli, and the presence of PELP1 in these fractions was analyzed by immunoblotting. Paxillin, lamin and nucleolin were used as cytoplamic, nuclear and nucleoli markers respectively.