PCR analysis to map GCR breakpoints.

<p>*PCR primers were designed to anneal to <i>LYS2</i> sequence upstream and downstream of the embedded Sμ fragment (<i>GTOP</i> or <i>GBTM</i>). See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004839#pgen-1004839-g001" target="_blank">Fig. 1A</a> for the approximate location of the PCR fragments analyzed. + indicates the presence of PCR product and − indicates the absence.</p><p>PCR analysis to map GCR breakpoints.</p>