PCR analysis and southern blot analysis of the transgenic maize.

A PCR detection of the gene bgl7Am in the genomic DNA of transgenic plant leaves of generation BC1. Lane 1, the plasmid PHP20754-bgl7Am as positive control; lane 2–9, the transgenic plants; lane 10, the non-transgenic Zheng58. B PCR detection of the gene actin. Lane 1, the plasmid PHP20754-bgl7Am; lane 2–9, the transgenic plants; lane 10, the non-transgenic Zheng58. C Southern blot analysis of bgl7Am in transgenic plants. The EcoRI and HindIII-digested genomic DNA was hybridized with the bgl7Am probe. Lane 1, the DIG-labeled molecular weight markers; lane 2–4, transgenic plants digested by EcoRI (arrowhead indicate the positive bands); lane 5, non-transgenic Zheng58 digested by EcoRI; lane 6, non-transgenic Zheng58 digested by HindIII; lane 7–9, transgenic plants digested by HindIII (arrowhead indicate the positive band); lane 10, PCR fragment of the bgl7Am as a positive control (arrowhead).