P38MAPK, ERK and PI3K pathway inhibitors block C5a-primed neutrophils for ANCA-induced degranulation.

ANCA-induced neutrophil degranulation was determined by measuring the lactoferrin concentrations in the supernatant of neutrophil degranulation reaction. Inhibition of p38MAPK, ERK and PI3K reduced ANCA-induced lactoferrin release. Bars represent mean±SD of 3 MPO-ANCA and 3 PR3-ANCA preparations, measured on neutrophils of 4 donors. T test was used to compare concentration of lactoferrin between groups.