Oxygen consumption of isolated mitochondria in Risp+/P224S mice.
Electron paths due to the combination of substrate and inhibitors used are indicated below each bar, as well as the sex (F for female, M for male). (A) Mitochondria isolated from liver of 3-month-old mice. All measurements were made in wild-type/heterozygote littermate pairs, and results are expressed as the effect of Risp heterozygosity relative to wild-type within each pair. (B) Mitochondria isolated from heart of 3-month-old mice. All measurements were made in wild type/heterozygote littermate pairs, and results are expressed as the effect of Risp heterozygosity relative to wild-type within each pair. (C) Mitochondria isolated from liver of 2-year-old mice. Due to the low probability of both members of wild-type/heterozygote littermate pairs surviving to this age, comparisons were made between the complete group of mice of each genotype. Actual values for (A) and (C) are shown in Table S2. Values for state 4 respiration and respiratory control ratio (state 3/state 4) are shown in Table S3. For all figures, sample sizes are shown superimposed on each bar. ‘*’ denotes p = 0.01 to 0.05 vs. 1.0 or wild-type control, ‘#’ p = 0.097 vs. 1.0.