Overexpression of Myc increases mutation frequency and decreases lifespan at 29

<p>°<b>C.</b> (A) Western blot from five adult heads at 29°C for three days from <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as Act<sup>TS</sup>>+) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as Act<sup>TS</sup>>Myc). Myc is induced ∼9-fold in this system (LiCOR quantitation). (B) <i>lacZ</i> mutation frequency of <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as “Act<sup>TS</sup>>+”) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as “Act<sup>TS</sup>>Myc”). Adults were placed at 29°C for 5 days before <i>lacZ</i> analyses to allow transgene expression. Black solid areas indicate frequency of point mutations and white areas indicate frequency of genome rearrangements. *indicates statistical significance of p<0.01 (student’s t-test). (C) Lifespan analyses of females of the genotype <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as “Act<sup>TS</sup>>+”) and of the genotype <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as “Act<sup>TS</sup>>Myc”). These lifespans are statistically significantly different from each other p<0.001 (Log-rank test).</p>