Outline of the auxiliary workflow used for lipidome data processing.

(A) A sequence of processing steps executed by the Orange software is used to compute the molar abundance of lipid species. The processing routine utilizes three input files: (a) the output file generated by the ALEX framework specifying lipid intensities, (b) a text file specifying sample information, and (c) a text file specifying internal standards and molar spike amounts. Seven processing steps are executed in order to compute the molar abundances of lipid species for all samples. The data processing generates an output file in database table format featuring the molar abundances of lipid species, originating intensity data, all accessory lipid features and all sample information. (B) Lipid class composition of cerebellum, hippocampus and S1BF from wild-type and PRG-1 knockout mice as automatically calculated and displayed using Tableau Software.