Osteoclast or osteoblast marker analysis.

A: C4-2B and PC-3 cells were treated with 100 ng/ml OPG or 10 ng/ml TGF-β for 24 hours. The expression of RANKL and CXCR-4 protein was measured by Western Blot analysis. B and C: RAW246.7 (B) and hFOB1.19 (C) cells in lower chambers were co-cultured with C4-2B (C4), PC-3 (PC), ARCaP_M (M) and ARCaP_E (E) prostate cancer cells in upper chambers for 48 hours. Western Blot analysis was conducted to measure the expression of osteoclast or osteoblast markers in RAW264.7 or hFOB1.19 cells.