OMV protein profiles of independent preparations.

Shown are protein profile comparisons of three independent OMV preparations from the NTHi strains 2019-R (A) and 3198-R (B). Samples (approx. 4 µg protein each) were separated by SDS-PAGE (12,6% gels) and protein bands were visualized according to Kang et al. [59]. Lines to the left indicate the molecular masses of the protein standards in kDa. Proteins identified by mass spectrometry from NTHi 2019-R OMVs are indicated with their respective position on the gel, protein identities and accession numbers on the right.