Nxf1^CAST does not suppress mRNA deficit in Atrn^mgL.

(A) Scale diagram of the mouse Atrn locus indicates the location and approximate size of the IAP insertion in mg^L allele and the Northern blot probe. (B) Northern blot analysis of brain poly(A)⁺ RNA (5 µg per lane) from non-mutant and mutant littermates shows reduced level of full-length Atrn mRNA in mg^L animals, independent of Nxf1 allele (B for C57BL/6, C for CAST/Ei). (C, D) Quantitative PCR (TaqMan) data shows nominal reduction in the abundance of spliced 5′ sequences, but ∼6-fold reduction of 3′ sequences, consistent with alternative splicing and 3′ end formation in the insertion, with no difference between Nxf1 genotypes. Error bars indicate standard deviation.