No Nef uptake into Jurkat by flow cytometry.

<p>293T cells were transfected with cDNA3 (Mock), GFP, Nef.GFP, or CD81-GFP plasmid and cultured in exosome-free medium for 3 days. Cell culture media were collected and removed of cell debris (Supernatants), or used to prepare crude exosomes (Exosomes) as described above. Jurkat (1 x 10<sup>5</sup>) were incubated with 100 μl crude exosomes (<b>A</b>) or 100 μl supernatants (<b>B</b>) for 3, 12, 24, or 48 hr. The cells were then washed with PBS multiple times and analyzed by flow cytometry. The data were representative of four independent experiments.</p>