Neutrophil recruitment following L. braziliensis inoculation.

Individual BALB/c mice were injected with L. braziliensis in the ear dermis. At different time points, cells were prepared and stained for Ly6G and analyzed by flow cytometry. (A) Representative dot plots and gating strategy of neutrophils. (B) Bar graph representation of the number of Ly6G⁺ cells in naïve mice (grey bars), in mice inoculated with saline (white bars) or inoculated with L. braziliensis (black bars). Data shown (mean ± SEM) are from one experiment representative of two. ***p <0.001, *p<0.05.