Figure_4.tif (268.15 kB)
Neutrophil lysis by S. epidermidis PSM peptides and culture filtrates of PSMδ-expression strains.
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posted on 2013-02-20, 23:01 authored by Gordon Y. C. Cheung, Kevin Rigby, Rong Wang, Shu Y. Queck, Kevin R. Braughton, Adeline R. Whitney, Martin Teintze, Frank R. DeLeo, Michael Otto(A) Neutrophil (PMN) lysis by synthetic, N-formylated PSM peptides at 10 µg/ml was determined by measuring release of LDH (incubation time, 1 h). PSMα3 (S. aureus) was used as a comparison at the same concentration. (B) Neutrophil lysis using supernatants (18-h cultures) of a PSMδ-over-expressing agr-negative (lacking intrinsic PSM production) and corresponding control strains (incubation time, 6 h). pTXpsmδ, pTX construct expressing PSMδ; pTX16, control plasmid. Strains were grown in TSB with 0.5% xylose and 12.5 µg/ml tetracycline. **, p<0.01, paired t-tests. Blue bars, PSMδ concentration in the culture filtrates relative to that in the 1457 wild-type (set to 100%).