NKCC1 antisense blocks TBS-induced positive shift in E_GABA in neonatal neurons.

In panel A, TBS-induced positive shift in E_GABA was observed 30 min following TBS in control cells. The positive shift was still observed in cells loaded with NKCC1 sense mRNA (panel B), while completely blocked in NKCC1 antisense mRNA loaded cells (panel C). Note the PSC conductance was not changed pre- and post TBS in both cases. In each panel, PSC records (on the top) were evoked at different holding potentials and I-V plots (on the bottom) represented corrected PSC amplitudes at various holding potentials. In all panels, the left PSC records are controls and the right ones are taken 30 min following TBS conditioning. Data in panels A, B & C were obtained from three different CA1 neurons from 3–5 day old rats and the holding potential for each individual cell was -60 mV. The whole-cell recording method was used in this study.