Figure_3.tif (397.31 kB)
NFAT5 and HIF-1α are independently up-regulated by hypoxia.
figure
posted on 2013-02-20, 02:59 authored by Sandra Villanueva, Cristian Suazo, Daniela Santapau, Francisco Pérez, Mariana Quiroz, Juan E. Carreño, Sebastián Illanes, Sergio Lavandero, Luis Michea, Carlos E. IrarrazabalHEK293 cells cultured at 300 mosmol were transfected with control (C), NFAT5 or HIF-1α siRNA. 48 hrs after transfection the cells were cultured in normoxia (N) or 8 hrs of hypoxia (H). A. Protein abundance of NFAT5 and HIF-1α were studied by Western blot in cells transfected with siRNA against NFAT5. B. Protein abundance of NFAT5 and HIF-1α were studied by Western blot in cells transfected with siRNA against HIF-1α. A representative picture is shown in the upper section. Bar graph represents Mean ± SEM. *, P<0.05; n = 5.
