NCI-H28 and REN cells express significant less amount of PAR<sub>1</sub> on plasma membrane.

<p>Cell surface PAR<sub>1</sub> expression was measured by ELISA using a polyclonal antibody which recognizes the N-terminus of PAR<sub>1</sub><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111550#pone.0111550-Paing1" target="_blank">[31]</a>. Antibody binding to fixed cells was detected by horseradish peroxidise-conjugated secondary antibody. Data represent the mean ± SEM of three independent experiments performed in triplicate. The differences in cell surface PAR<sub>1</sub> expression between Ctrl (Met-5A cells) and MPM (NCI-H28 and REN) cellswere significant (***P≤0.001) by one-way ANOVA followed by Bonferroni’s multiple comparison test.</p>