Mycobacteria specific intracellular IFN-γ production in CD4⁺ and CD8⁺ T spleen cells of mice vaccinated with MGM1991, HMA or BCG vaccine at 4 weeks after immunization.

Upper panel (A): ICS staining of CD4⁺ spleen cells from C57BL/6 mice vaccinated 4 weeks before with 5x10⁴ CFU of MGM1991, HMA or M. bovis BCG by the subcutaneous route or from unvaccinated (naïve) mice. Mean % of positive cells in each quadrant (of 3 mice tested individually in each group).

Lower 2 panels (B): ICS staining of CD8⁺ spleen cells from C57BL/6 mice vaccinated 4 weeks before with 5x10⁴ CFU of MGM1991, HMA or M. bovis BCG by the subcutaneous route or from unvaccinated (naïve) mice. Results show results obtained in 2 individual mice.

^*p<0,05 MGM1991 or HMA vs BCG; ^ŦP<0,05 MGM1991 vs HMA; (Mann-Whitney test).