Myc overexpression increases the number of γ-H2A.X positive cells.

(A, B) Wing imaginal disc of genotype ap-Gal4, UAS-GFP/+; UAS-p35/+. (C, D) Wing imaginal disc of genotype ap-Gal4, UAS-GFP/+; UAS-p35/UAS-Myc. GFP channel is shown in A and C, whereas γ-H2A.X staining is shown in B and D. (E) Quantitation of the number of γ-H2A.X positive cells from ap-Gal4, UAS-GFP/+; UAS-p35/+ wing discs (shown as ap>p35) and ap-Gal4, UAS-GFP/+; UAS-p35/UAS-Myc (shown as ap>Myc+p35). The number of γ-H2A.X positive cells within the ap>Gal4 expressing region (shown in GFP channel) from at least 10 imaginal discs was quantitated, and the error bars represent standard error. *indicates statistical significance of p<0.01 (student’s t-test). (F) Western blot analyses showing levels of Myc and the loading control γ-tubulin of wing imaginal discs of the genotypes ap-Gal4, UAS-GFP/+; UAS-p35/+ (labeled as p35) and ap-Gal4, UAS-GFP/+; UAS-p35/UAS-Myc (labeled as Myc+p35). Eight discs were loaded per lane. Myc is overexpressed 5.3-fold as quantitated using LiCOR software and normalized to the level of γ-tubulin (average of three Westerns).