Myc overexpression in the adult increases mutation frequency and reduces lifespan at 25

<p>°<b>C.</b> (A) Western analyses showing levels of Myc and the loading control γ-tubulin from four female adult heads of the genotypes <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as “Act<sup>TS</sup>>+”) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as “Act<sup>TS</sup>>Myc”). Adults were placed at 25°C for 10 days before Western analyses. (B) <i>lacZ</i> mutation frequency of females of genotype <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as “Act<sup>TS</sup>>+”) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as “Act<sup>TS</sup>>Myc”). Adults were placed at 25°C for 10 days before <i>lacZ</i> analyses. *indicates statistical significance of p<0.001 (student’s t-test). (C) Lifespan analyses at 25°C of females of the genotypes <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as “Act<sup>TS</sup>>+”) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as “Act<sup>TS</sup>>Myc”). These lifespans are statistically significantly different from each other p<0.001 (Log-rank test). (D) Lifespan analyses at 25°C of females of the genotypes <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-p35 (shown as “Act<sup>TS</sup>>p35”) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc, UAS-p35 (shown as “Act<sup>TS</sup>>Myc+p35”). These are statistically significantly different (p<0.001, Log-rank test).</p>