MyD88 does not play a central role in MALP-2-induced HO-1 expression.

<p><b>A.</b> THP-1 cells were transiently transfected with a dominant negative plasmid encoding MyD88 (DN-MyD88) or empty vector (pDeNy, lane 2). Cell lysates were prepared, and samples were immunoblotted with an anti-HO-1 antibody. <b>B.</b> Cells were transfected with MyD88 siNRA or control siRNA, and then treated with MALP-2 (5 ng/ml) for 16 h. Expression of MyD88 and HO-1 were analyzed by Western blotting. All values are expressed as means ± SEM obtained from three independent experiments. **, P<0.01 for significant difference between compared groups.</p>