Figure_4.tif (107.59 kB)
MyD88 does not play a central role in MALP-2-induced HO-1 expression.
figure
posted on 2014-07-31, 03:00 authored by Xiaoxing You, Liangzhuan Liu, Yanhua Zeng, Ranhui Li, Jun He, Xiaohua Ma, Chuanhao Jiang, Cuiming Zhu, Liesong Chen, Minjun Yu, Guangli Ou, Yimou WuA. THP-1 cells were transiently transfected with a dominant negative plasmid encoding MyD88 (DN-MyD88) or empty vector (pDeNy, lane 2). Cell lysates were prepared, and samples were immunoblotted with an anti-HO-1 antibody. B. Cells were transfected with MyD88 siNRA or control siRNA, and then treated with MALP-2 (5 ng/ml) for 16 h. Expression of MyD88 and HO-1 were analyzed by Western blotting. All values are expressed as means ± SEM obtained from three independent experiments. **, P<0.01 for significant difference between compared groups.
