Multivariate analysis using PCA of the parameters in tables 1 and 2 (with BMI and age excluded) using two significant principal components based on eigenvalue criteria (>2).

A. Females with ALS overlapped with female controls in the measured parameters. (t [1], 34% of variation vs. t [2], 20% of variation; R2 = 0.67; Q2 = .25). B) The male ALS group was shifted from the male controls in the measured parameters. (t [1], 36% of variation vs. t [2], 15% of variation; R2 = 0.51; Q2 = 0.22).